Luminescent ATP Cell Viability Assay Kit I

ATP is an important source of energy within cells and can be used to measure the level of metabolism in cells. ATP levels are also positively correlated with the number of viable cells. Therefore, ATP levels can be used to measure the number of viable cells.

Luminescent ATP Cell Viability Assay Kit I is a kit that uses firefly luciferase to detect cell viability. The reagent provided in this kit contains thermal-stable firefly luciferase and a highly purified substrate luciferin. This reagent can lyse cells and release ATP, which then produces a stable luminescence signal catalyzed by luciferase. The intensity of its luminescence signal is proportional to the number of viable cells.

The reagent provided in this kit is a ready-to-use solution and does not require cell lysis in advance, making it very convenient to use. Compared to other cell viability reagents, such as MTT, CCK-8, Alamar Blue, and Calcein-AM, this reagent offers higher sensitivity and shorter detection times. In addition, the reagent has good linearity in 10-30,000 cells. This kit has been tested to be as effective as CellTiter-Glo^® Luminescent Cell Viability Assay (CTG).

Figure 1: Schematic diagram of the detection of this kit

Figure 2: Validation diagram of this kit. The left picture shows the effect of this kit and similar foreign products (Competitor P) for detecting cell proliferation, and the right picture shows the effect of this kit on the ATP standard curve