Malachite Green Phosphate Assay Kit
The Malachite Green Phosphate Assay Kit is a kit for the detection of phosphate concentration. The principle is that under acidic conditions, malachite green, molybdate and free inorganic orthophosphate can form a green phosphomolybdate complex, and the absorbance of this green phosphomolybdate complex at 650 nm is proportional to the concentration of free inorganic phosphate in solution.
This kit can quickly, conveniently and sensitively determine free inorganic phosphate in solution, so it is often used for the determination of phosphate concentration in solution and the ATPase activity. However, this kit is only suitable for the determination of free phosphate in solution. For the bound phosphate, such as binding to lipids or proteins, certain methods must be used for release before detection. The kit has a good linear relationship in the range of 0-10 nmol during detection.
Figure 1: Reference standard curve of this kit
| Components | K2245-500 T | K2245-1000 T | K2245-2500 T |
| Malachite Green Reagent A | 5 mL | 10 mL | 25 mL |
| Malachite Green Reagent B | 10 mL | 20 mL | 50 mL |
| Phosphate Standard (10 mM) | 400 μL | 800 μL | 2 mL |
Store the kit at 4°C, stable for 1 year. Malachite Green Reagent B should be stored away from light. | |||