Nile Red
Nile Red, is a fluorescent stain specific for the detection of intracellular lipid droplets in hydrophobic environment, but show minimal fluorescence in aqueous media. Nile Red can be applied for staining intracellular lipids, lysosomal phospholipid inclusions and hydrophobic domains of proteins, and detected by fluorescence microscopy and flow cytofluorometry. In all organic solvents, Nile Red is intensely fluorescent, with colors ranging from golden yellow to red.
Reference:
1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973.
| Storage | Store at -20°C |
| M.Wt | 318.37 |
| Cas No. | 7385-67-3 |
| Formula | C20H18N2O2 |
| Solubility | ≥2.56 mg/mL in DMSO; insoluble in EtOH; insoluble in H2O |
| Chemical Name | 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one |
| SDF | Download SDF |
| Canonical SMILES | CCN(C1=CC2=C(N=C3C4=CC=CC=C4C(C=C3O2)=O)C=C1)CC |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Monkey aortic smooth muscle cells and mouse peritoneal macrophages, induced by acetylated low density lipoprotein |
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Reaction Conditions |
100 ng/ml Nile Red |
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Applications |
Better selectivity for cytoplasmic lipid droplets was obtained when the cells were viewed for yellow-gold fluorescence (excitation, 450-500 nm; emission, greater than 528 nm) rather than red fluorescence (excitation, 515-560 nm; emission, greater than 590 nm). |
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Note |
The technical data provided above is for reference only. |
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References: 1. Greenspan P, Mayer EP, Fowler SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. Journal of Cell Biology, 1985, 100(3): 965-973. |
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Quality Control & MSDS
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Chemical structure
