Phosphatase Inhibitor Cocktail (2 Tubes, 100X)
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Protein phosphorylation is an important covalent posttranslational modification that changes the conformation of proteins and regulates the function, location and specific binding of target proteins. Many cellular processes are regulated by reversible phosphorylation of proteins, and 30% of proteins may be phosphorylated in their presence.
Due to the balance of endogenous protein production and degradation, its cell level is stable under stable environmental conditions. The crude cell extract contains many endogenous enzymes, such as phosphatase and protease, which degrade and alter the proteins in the extract. The best way to increase complete protein production is to add inhibitors of these enzymes.
More inhibitors mean better phosphorylation protection. We offer two tubes of inhibitors with different solution forms to help you better inhibit phosphatase.
Tube A (phosatase Inhibitor Cocktail 1, Catalog No. K1012) inhibits l-isozyme of alkaline Phosphatase and serine/threonine protein phosphatases, such as protein Phosphatase 1 and 2A (PP1 and PP2A). Tube A contains Cantharidin, Bromotetramisole and Microcystin LR, which are provided in the solution of DMSO.
Tube B (phosatase Inhibitor Cocktail 1, Catalog No. K1013) inhibits tyrosine protein Phosphatase, acid Phosphatase and alkaline Phosphatase. Tube B contains Sodium orthovanadate, Sodium molybdate, Sodium tartrate, Imidazole and Sodium Fluoride, with ddH2O box solution is provided.
| Thawed at room temperature, then diluted to solution samples (such as cell lysates or tissue extracts) at 1:100 (v/v) before the experiment, add A tube and B tube successively: add A tube, mix well; Add B tube, mix well (do not mix A tube and B tube and then add together). |
| WB，Co-IP，pull-down，IF，IHC，kinase assay and etc. |
Related Biological Data
Related Biological Data
| Tube # | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| N1686 | Cantharidin | Protein phosphatase 1/2a inhibitor | Natural Products | 56-25-7 | O=C([C@@]1(C([H])([H])[H])[C@]2(C([H])([H])[H])[C@@]3([H])O[C@]1([H])C([H])([H])C3([H])[H])OC2=O | |
| B4750 | (-)-p-Bromotetramisole Oxalate | ALP inhibitor | Others|Others | 62284-79-1 | BrC(C=C1)=CC=C1[C@H]2N=C3SCCN3C2.OC(C(O)=O)=O | |
| B3698 | Microcystin-LR | Protein phosphatase PP1/PP2A inhibitor | Chromatin/Epigenetics|Protein Ser/Thr Phosphatases | 101043-37-2 | CC1C(NC(=O)C(NC(=O)C(C(NC(=O)C(NC(=O)C(NC(=O)C(=C)N(C(=O)CCC(NC1=O)C(=O)O)C)C)CC(C)C)C(=O)O)C)CCCN=C(N)N)C=CC(=CC(C)C(CC2=CC=CC=C2)OC)C | |
| B (100X in ddH2O) | ||||||
| A8524 | Sodium Orthovanadate | PTP inhibitor | Membrane Transporter/Ion Channel|ATPase | 13721-39-6 | [O-][V](=O)([O-])[O-].[Na+].[Na+].[Na+] | |
| B7843 | Sodium molybdate | Acid and phosphatase inhibitor | 7631-95-0 | [O-][Mo](=O)([O-])=O.[Na+].[Na+] | ||
| B7844 | Sodium tartrate | Acid phosphatase inhibitor | 868-18-8 | C(C(C(=O)[O-])O)(C(=O)[O-])O.[Na+].[Na+] | ||
| B7845 | Imidazole | Alkaline phosphatase inhibitor | 288-32-4 | C1=CN=CN1 | ||
| B7846 | sodium fluoride | Acid phosphatase inhibitor/td> | 7681-49-4 | [F-].[Na+] | ||
| Store at -20°C for about 12 months. Store at 2-8°C for about 2 months. | ||||||