PreScission Protease (PSP)
- 1. Xiaoying Wei, Zhishuo Wang, et al. "Human TDP1, APE1 and TREX1 repair 3′-DNA–peptide/protein cross-links arising from abasic sites in vitro." Nucleic Acids Res. 2022 Apr 22;50(7):3638-3657. PMID: 35349719
- 2. Jennifer Carlson. "Regulation of epigenetics and development by the xenobiotic/oxidative stress response factor Keap1 in Drosophila." University of Minnesota. July 2022.
PreScission Protease is a recombinant protease that combines human rhinovirus type 14 3C protease and GST. This protease can specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between Gln and Gly amino acid residues. Its substrate recognition and cleavage depend not only on the primary structure of the recombinant protease but also on its secondary structure and tertiary structure. It specifically isolates the heterologous proteins expressed by the vectors such as pGEX-6p series from its fused GST label to obtain heterologous target proteins with higher purity.
| Components | size | size | size |
| PreScission Protease | 100 U | 200 U | 500 U |
| 10X Cleavage Buffer | 1 mL | 1 mL x 2 | 1 mL x 3 |
Dry ice transport. -80°C for long-term storage, valid for 2 years; small sub-pack -20°C for 6 months. 10X Cleavage Buffer was stored at -20°C. Avoid repeated freeze-thaw! | |||
Sterile colorless liquid
used at low temperatures (4°C)
specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues.