Protease Inhibitor Cocktail (EDTA-Free,100X in DMSO)
Endogenous proteins are produced and degraded in equilibrium and therefore their cellular level is stable under stable environmental conditions. Crude cell extracts contain many endogenous enzymes capable of degrading proteins in the extract, such as phosphatases and proteases. When proteins are extracted from cells and tissues in vitro, protein production is significantly inhibited and degradation increases. The best way to increase intact protein production is to add inhibitors of those enzymes that are known to be present. The protease inhibitor cocktail is used in cell lysates or tissue extracts to improve protein stability.
The broad specificity of this Cocktail makes it a versatile protease inhibitor mix and is suitable for purification of histidine-tagged proteins. The protease inhibitor cocktail contains several important components, including AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Phosphoramidon, and Pepstatin A, which are broadly specific for serine, cysteine, acid proteases, aminopeptidases, and metalloproteases. The product is provided as a DMSO ready-to-use solution and can be used for Western Blot, Co-IP, pull-down, IF, IHC, Kinase assay and other experiments.
| Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | |
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| A2571 | Pepstatin A | Aspartic protease inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| B4790 | Phosphoramidon Disodium Salt | Metalloprotease inhibitor | Proteases | Metalloprotease | 164204-38-0 | O=C([O-])[C@@H](NC([C@@H](NP(O[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O)([O-])=O)CC(C)C)=O)CC2=CNC3=C2C=CC=C3.[Na+].[Na+] |
Stored at -20°C for 12 months. | |||||