Reactive Oxygen Species Assay Kit
Catalog No.
K2065
A kit that uses the fluorescent probe DCFH-DA to quantitatively assess reactive oxygen species (ROS)
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Reactive Oxygen Species Assay Kit is a kit that uses the fluorescent probe DCFH-DA to quantitatively assess reactive oxygen species (ROS) in live cells. DCFH-DA is a cell-permeable probe, which is deacetylated by cellular esterases to a non-fluorescent DCFH. DCFH then is oxidized by ROS into highly fluorescent DCF. The fluorescence intensity of DCF is proportional to the ROS levels. This kit also provides Rosup as a positive control for ROS generation. The concentration of Rosup is 50 mg/mL.
Figure 1: The efficiency of this kit for ROS detection
| Components | K2065-100 T | K2065-500 T |
| DCFH-DA (10 mM) | 0.1 mL | 0.5 mL |
| Rosup | 1 mL | 5 mL |
This kit should be stored at -20°C, stable for 1 year. DCFH-DA should be stored at -20°C away from light, and avoid repeated freeze/thaw cycles. | ||