Recombinant Human B cell Activating Factor Rececptor/TNFRSF13C
B Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation.
Reference
1. Castigli E, Wilson SA, Scott S, et al. 2005. J Exp Med, 201: 35-9
2. Nakajima K, Itoh K, Nagatani K, et al. 2007. Scand J Rheumatol, 36: 365-72
3. Zhu XJ, Shi Y, Peng J, et al. 2009. Blood, 114: 5362-7
4. Thibault-Espitia A, Foucher Y, Danger R, et al. 2012. Am J Transplant, 12: 2754-62.
| Gene ID | 115650 |
| Accession # | Q96RJ3 |
| Alternate Names | BAFFR, TNFRSF13C |
| Source | Escherichia coli. |
| M.Wt | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence | MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 8.0, 500 mM NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0 μg/ml in the presence of 1.0 μg/ml of rHuBAFF. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95 % by reduced SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rHuBAFF-R as determined by LAL method.