Recombinant Human IFN-beta
Interferon beta (IFN-beta), also known as fibroblast IFN, is a secreted, approximately 22 kDa member of the type I interferon family of molecules [1]. Mature human IFN-beta shares 47% and 46% amino acid sequence identity with the mouse and rat proteins, respectively. Fibroblasts are the major producers of IFN-beta, but it can also be produced by dendritic cells, macrophages, and endothelial cells in response to pathogen exposure [2]. It is trans -criptionally regulated by TRAF3, IRF3, IRF7, and NF-kappa B [3]. Following secretion, IFN-beta signals through the heterodimeric IFN-alpha / beta Receptor and activates the JAK/STAT signaling pathway [4-7]. IFN-beta -deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS) [8]. Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS [9-13].
Reference
[1]. González-Navajas, J.M. et al. (2012) Nat. Rev. Immunol. 12:125.
[2]. Reder, A.T. and X. Feng (2013) Front. Immunol. 4:281.
[3]. Schafer, S.L. et al. (1998) J. Biol. Chem. 273:2714.
[4]. Honda, K. et al. (2006) Nat. Rev. Immunol. 6:644.
[5]. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
[6]. Fasler-Kan, E. et al. (1998) Eur. J. Biochem. 254:514.
[7]. Matikainen, S. et al. (1999) Blood 93:1980.
[8]. Teige, I. et al. (2003) J. Immunol. 170:4776.
[9]. Shinohara, M.L. et al. (2008) Immunity 29:68.
[10]. Guo, B. et al. (2008) J. Clin. Invest. 118:1680.
[11]. Ramgolam, V.S. and S. Markovic-Plese (2010) Endocr. Metab.
[12]. Martín-Saavedra, F.M. et al. (2008) Mol. Immunol. 45:4008.
[13]. Inoue M. and M.L. Shinohara (2013) Immunology 139:11.
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Accession # |
P01574 |
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Alternate Names |
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta |
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Source |
Human embryonic kidney cell, HEK293-derived human IFN-beta protein |
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Protein sequence |
Met22-Asn187 |
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M.Wt |
20.0 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus.The EC50 for this effect is 1-10 ng/mL |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.