Recombinant Human IL-10
Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includesIL-19, IL-20, IL-22, IL-24, and IL-26/AK155 [1, 2]. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts [2-5]. Mature human IL-10 shares 72%-86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells [6, 7]. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer [6, 8, 9]. The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 [10]. IL-10 R beta does not bind IL-10 by itself but is required for signal transduction [1]. IL-10 R beta also associates with IL-20 R alpha, IL-22R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29[11-13]. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation [14-16]. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses [2].
Reference
[1]. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
[2]. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
[3]. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
[4]. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
[5]. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
[6]. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
[7]. Hsu, D.-H. et al. (1990) Science 250:830.
[8]. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
[9]. Syto, R. et al. (1998) Biochemistry 37:16943.
[10]. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
[11]. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
[12]. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
[13]. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
[14]. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
[15]. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
[16]. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.
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Accession # |
P22301 |
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Alternate Names |
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A |
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Source |
Human embryonic kidney cell, HEK293-derived human IL-10 protein |
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Protein sequence |
Ser19-Asn178 |
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M.Wt |
18.6 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The EC50 for this effect is 50-150 pg/mL. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.