Recombinant Human IL-12
IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.
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Gene ID |
3592 |
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Accession # |
P29459 |
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Alternate Names |
Interleukin-12, Natural killer cell stimulatory factor chain 2, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40. |
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Source |
Spodoptera frugiperda, Sf21 (baculovirus) |
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M.Wt |
Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits. |
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AA Sequence |
HuIL-12 p40: Ile23 - Ser328; Accession # P29460 HuIL-12 p35: Arg23 - Ser219; Accession # P29459 |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. - 1 month, 2 to 8°C under sterile conditions after reconstitution. - 3 months, -20 to -70°C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.2. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriately buffered solutions. |
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Biological Activity |
Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1×104 units/μg, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544). |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & MSDS
- View current batch:
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Purity >97% by SDS-PAGE.
- Datasheet
Endotoxin: Less than 1.0 EU/µg of rHuIL-12 as determined by LAL method.