Recombinant Human IL-19
Human Interleukin-19 (IL-19) is encoded by the IL19 gene, which is located on the chromosome 1. The protein belongs to the IL-10 family that includes IL-10, IL-20, IL-22, IL-24, and IL-26. As a monomer made of seven amphipathic helices, IL-19 has a helical bundle and shares the same cell surface receptor (IL-20R) with IL-20 and IL-24. It may play some important roles in inflammatory responses because it up-regulates IL-6 and TNF-alpha and induces apoptosis.
Reference:
1. Chang C, Magracheva E, Kozlov S, et al. 2003. J Biol Chem, 278: 3308-13.
2. Gallagher G, Dickensheets H, Eskdale J, et al. 2000. Genes Immun, 1: 442-50.
3. Liao YC, Liang WG, Chen FW, et al. 2002. J Immunol, 169: 4288-97.
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Gene ID |
29949 |
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Accession # |
Q9UHD0 |
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Alternate Names |
Interleukin-19, Melanoma Differentiation-associated Protein-like Protein, NG.1 |
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Source |
Escherichia coli. |
|
M.Wt |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
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AA Sequence |
LRRCLISTDM HHIEESFQEI KRAIQAKDTF PNVTILSTLE TLQIIKPLDV CCVTKNLLAF YVDRVFKDHQ EPNPKILRKI SSIANSFLYM QKTLRQCQEQ RQCHCRQEAT NATRVIHDNY DQLEVHAAAI KSLGELDVFL AWINKNHEVM SSA |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. - 1 month, 2 to 8°C under sterile conditions after reconstitution. - 3 months, -20 to -70°C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAC to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human IL-20Rα and human IL-20Rβ co-transfected murine BaF3 pro-B cells is less than 1.5 ng/ml, corresponding to a specific activity of > 6.7×105 IU/mg. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & MSDS
- View current batch:
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Purity >95% by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/µg of rHuIL-19 as determined by LAL method.