Recombinant Human IL-3
Interleukin-3 (IL-3) is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Reference
[1]. Meyer CG, et al. (2011) Hum Mol Genet. 20(6):1173-81.
[2]. Zambrano A, et al. (2010) Curr Alzheimer Res. 7(7):615-24.
[3]. Dentelli P, et al. (2011) Oncogene. 30(50):4930-40.
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Accession # |
AAC08706 |
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Alternate Names |
Human IL3; IL3; IL-3; recombinant IL3; interleukin-3; Mast cell growth factor |
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Source |
Human embryonic kidney cell, HEK293-derived human IL3 protein |
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Protein sequence |
Ala20-Phe152 |
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M.Wt |
15.1 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 0.01-0.08 ng/mL. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.