Recombinant Human IL-4
Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a secreted protein that belongs to the IL-4 / IL-13 family [1-3]. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four α-helix structure [4]. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities [5-7]. IL-4 exerts its effects through two receptor complexes [8, 9]. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and the common γ chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 Rα and IL-13 Rα1.The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils [1, 2]. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naive CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells [10-13].
Reference
[1]. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
[2]. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
[3]. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
[4]. Redfield, C. et al. (1991) Biochemistry 30:11029.
[5]. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
[6]. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
[7]. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
[8]. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
[9]. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
[10]. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
[11]. Corthay, A. (2006) Scand. J. Immunol. 64:93.
[12]. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
[13]. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
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Accession # |
P05112 |
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Alternate Names |
Human IL4; hIL-4, recombinant IL4, interleukin 4, BCGF1 Protein |
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Source |
Human embryonic kidney cell, HEK293-derived human IL4 protein |
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Protein sequence |
His25-Ser153 |
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M.Wt |
15 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The EC50 for this effect is 0.01-0.08 ng/mL. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.