Recombinant Human Interleukin-3 Receptor alpha/CD123 Fc Chimera Protein, Insect Cells Derived
Interleukin-3 receptor (IL-3 R) is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (IL-3 Rα or CD123) and a 120-140 kDa IL-3 R beta subunit (IL-3 Rβ or CD131). IL-3Rα is a glycoprotein member of the hematopoietic receptor superfamily. IL-3 Rα binds IL-3 with relatively low affinity. In the presence of IL-3 Rβ, however, IL-3 R alpha has a much higher affinity for IL-3.Emerging studies demonstrate that CD123, the IL-3 Rα, is highly expressed in leukemic stem cells (LSCs), while not normal hematopoietic stem cells (HSCs), and associates with treatment response, minimal residual disease (MRD) detection and prognosis. Furthermore, CD123 is an important marker for the identification and targeting of LSCs for refractory or relapsed leukemia.
| Source | Insect Cell |
| M.Wt | Approximately 60.3 kDa on SDS-PAGE under reducing conditions, containing 529 amino acids. |
| AA Sequence | AGMGTKEDPN PPITNLRMKA KAQQLTWDLN RNVTDIECVK DADYSMPAVN NSYCQFGAIS LCEVTNYTVR VANPPFSTWI LFPENSGKPW AGAENLTCWI HDVDFLSCSW AVGPGAPADV QYDLYLNVAN RRQQYECLHY KTDAQGTRIG CRFDDISRLS SGSQSSHILV RGRSAAFGIP CTDKFVVFSQ IEILTPPNMT AKCNKTHSFM HWKMRSHFNR K |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0, with 0.02 % Tween-20. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Testing in progress. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95 % by SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 0.01 EU/μg of rHuIL-3 Rα/CD123-Fc as determined by LAL method.