Recombinant Human Interleukin-7 GMP
Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Murine IL-7 has approximately 65 % amino acid sequence identity with human IL-7 and both proteins exhibit cross-speciesactivity. IL-7 as an immunotherapy agent has been examinedin many human clinical trials for various malignancies and during HIV infection.
Reference
1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6
2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2
3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601
4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80
5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904
6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.
| Gene ID | 3574 |
| Accession # | P13232 |
| Alternate Names | LP-1, pre-B cell factor |
| Source | Escherichia coli. |
| M.Wt | Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| AA Sequence | DCDIEGKDGK QYESVLMVSI DQLLDSMKEI GSNCLNNEFN FFKRHICDAN KEGMFLFRAA RKLRQFLKMN STGDFDLHLL KVSEGTTILL NCTGQVKGRK PAALGEAQPT KSLEENKSLK EQKKLNDLCF LKRLLQEIKT CWNKILMGTK EH |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Full biological activity when compared with standard protein by stimulating proliferation of PHA-activated human peripheral blood mononuclear cell (PBMC). The specific activity of recombinant human IL-7 is > 1.0 × 108Units/mg, which is calibrated against rHuIL-7 WHO reference reagent (NIBSC code: 90/530). |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 0.01 EU/μg of rHuIL-7 GMP as determined by LAL method.