Recombinant Human R-Spondin 1
R-Spondin 1 (RSPO1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that shares ~40% amino acid (aa) identity with three other R-Spondin family members [1, 2]. All R-Spondins regulate Wnt/ beta-Catenin signaling but have distinct expression patterns [1-3]. Human R-Spondin 1 (aa21-263) shares 89%, 87%, 92%, 91%, 91% and 89% aa identity with mouse, rat, horse, dog, goat, and cow RSPO-1, respectively. R-Spondin 1 competes with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors, Kremen and LRP-6, reducing their DKK-1-mediated internalization [4]. However, reports are mixed on whether R-Spondin 1 binds LRP-6 directly [4-6]. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development [3, 7]. Interest in R-Spondin 1 as a cell culture supplement has grown with the expansion of the organoid field. R-Spondin 1 is widely used in organoid cell culture workflows as a vital component that promotes both growth and survival of 3D organoids [8]. Structurally similar to other R-Spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta-catenin stabilization [2, 9]. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus [10]. Potential isoforms of 200 and 236 aa have an alternate, shorter N-terminus or are missing aa 146-208, respectively [11].
Reference
[1]. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
[2]. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
[3]. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
[4]. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
[5]. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
[6]. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
[7]. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
[8]. Drost and Clevers. (2018) Nature Reviews Cancer 18:407.
[9]. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
[10]. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
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Accession # |
Q2MKA7 |
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Alternate Names |
Cristin 3; HRspo1; roof plate-specific spondin; RSPO1; RSpondin 1; R-Spondin 1 |
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Source |
Human embryonic kidney cell, HEK293-derived human R-Spondin 1 protein |
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Protein sequence |
Ser21-Ala263 |
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M.Wt |
25.6 kDa |
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Appearance |
Solution protein. |
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Stability & Storage |
Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 °C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The EC50 for this effect is 1-10 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.