Recombinant Human Transforming Growth Factor - beta 2
Catalog No.
P1423
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TGF-β2 is a pleiotropic cytokine that regulates immune function, cellular proliferation, and epithelial-mesenchymal transition. It shows cross-species activity in the development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems. Latent TGF-β is activated by proteolytic cleavage of the mature cytokine from the latency-associated peptide. TGF-β2 signaling involves the accessory receptor Betaglycan, TGF-β RII, and a type I TGF-β receptor, resulting in the activation of Smad signal transduction.
| Source | Mouse myeloma cell line, NS0 |
| M.Wt | Apparent molecular mass of 24 kDa in SDS-PAGE under non-reducing conditions, 12 kDa under reducing conditions, a disulfide-linked homodimer of two 112 amino acid glycosylated polypeptide chains. |
| AA Sequence | Ala303-Ser414; Accession # P61812 |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from 0.2 μm filtered concentrated solution in 35 % Acetonitrile and 0.1 % TFA. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile 4 mM HCl to a concentration of 0.1 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions. |
| Biological Activity | Measured by its ability to inhibit the IL-4-dependent proliferation of HT2 mouse T cells. The ED50for this effect is 0.025-0.25 ng/mL. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE analyses.
- Datasheet
Endotoxin: Less than 0.1 EU/μg of rHuTGF-β2 as determined by LAL method.