Recombinant Mouse IL-1 beta/IL-1F2, Tag Free
Interleukin 1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 17% amino acid (aa) identity in mouse. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects [1-4]. The mouse IL-1 beta cDNA encodes a 269 aa precursor. A 117 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine [5, 6]. The17 kDa mature mouse IL-1 beta shares 90% aa sequence identity with cotton rat and rat and 65%-78% identity with canine, equine, feline, human, porcine, and rhesus IL-1 beta.
Reference
[1]. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
[2]. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
[3]. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
[4]. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
[5]. Gray, P.W. et al. (1986) J. Immunol. 137:3644.
[6]. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
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Accession # |
NP_032387 |
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Alternate Names |
IL1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1-beta |
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Source |
Human embryonic kidney cell, HEK293-derived mouse IL-1 beta/IL-1F2 protein |
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Protein sequence |
Val118-Ser269 |
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M.Wt |
17.5 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -7°C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 1-4 pg/mL. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.