Recombinant Mouse IL-5, Tag Free
Interleukin-5 (IL-5) is a secreted glycoprotein that belongs to the alpha -helical group of cytokines [1 - 3]. Unlike other family members, it is present as a covalently linked antiparallel dimer [4, 5]. The cDNA for mouse IL-5 encodes a signal peptide and a 113 amino acid (aa) mature protein. Mature mouse IL-5 shares 70%, 94%, 58%, 66%, 59% and 63%, aa sequence identity with human, rat, canine, equine, feline and porcine IL-5, respectively, and shows cross-reactivity with human IL-5 receptor. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, Reed-Sternberg cells in Hodgkin's disease, and IL-2-stimulated invariant natural killer T cells (iNKT) [1 - 3, 6 - 8]. IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow [1, 6, 9, 10]. The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal-transducing subunit, beta c [3, 6, 11]. IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor [12].
Reference
[1]. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
[2]. Elsas, P.X. and M. I. G. Elsas (2007) Curr. Med. Chem. 14:1925.
[3]. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
[4]. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
[5]. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
[6]. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
[7]. Lalani, T. et al. (1999)Ann. Allergy Asthma Immunol. 82:317.
[8]. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
[9]. Clutterbuck, E. J. et al. (1989) Blood 73:1504.
[10]. Cameron, L. et al. (2000) J. Immunol. 164:1538.
[11]. Tavernier, J. et al. (1991) Cell 66:1175.
[12]. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
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Accession # |
P04401 |
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Alternate Names |
IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5 |
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Source |
HHuman embryonic kidney cell, HEK293-derived mouse IL-5 protein |
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Protein sequence |
Met21-Gly133 |
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M.Wt |
13.1 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 17-35.6 pg/mL. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.