Recombinant Mouse IL25/IL17E, Tag Free
IL-25, which is also known as IL-17E, promotes Th2-biased immune responses. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. IL-25 is an important mediator of allergic reactions and protection against intestinal parasites [1, 2]. Mature mouse IL-25 shares 80% and 91% amino acid sequence identity with human and rat IL-25, respectively [3, 4]. During helminth infections and allergic reactions IL-25 is locally up-regulated in intestinal and airway epithelial cells, atopic dermatitis skin lesions, and local Th2 cells, eosinophils, and basophils [4-9]. It binds to IL-17RB but also requires IL-17 RA to exert its activity [3, 8, 10]. IL-25 acts on a variety of cell types which respond with increased production of Th2 cytokines (e.g. IL-4, IL-5, IL-13) and reduced production of Th1 and Th17 cytokines (e.g. IFN- gamma, IL-12, IL-23, IL-17A, IL-17F) [4-6, 8, 9]. Airway IL-25 can be activated by MMP-7, a protease that is up-regulated in airway epithelium in response to allergen exposure . Cleaved IL-25 shows enhanced binding to IL-17 RB and stronger induction of Th2 cytokines . The Th2 cytokines, in turn, trigger expansion of Th2 memory cells and anti-inflammatory M2 macrophages, increased eosinophil mobilization and activation, and dendritic cell migration [4, 6, 9]. These actions promote protective anti-helminth immune responses [4, 5] as well as allergic inflammation and airway hyperreactivity[11] . The IL-25 induced suppression of Th1 and Th17 cytokines limits Th17 cell expansion and disease pathology in autoimmunity and colitis[12].
Reference
[1]. Saadoun, D. et al. (2011) Curr. Pharm. Des. 17:3781.
[2]. Iwakura, Y. et al. (2011) Immunity 34:149.
[3]. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
[4]. Fort, M.M. et al. (2001) Immunity 15:985.
[5]. Zhao, A. et al. (2010) J. Immunol. 185:6921.
[6]. Suzukawa, M. et al. (2012) J. Immunol. 189:3641.
[7]. Corrigan, C.J. et al. (2011) Proc. Natl. Acad. Sci. USA 108:1579.
[8]. Petersen, B.C. et al. (2012) Nat. Med. 18:751.
[9]. Wang, Y.-H. et al. (2007) J. Exp. Med. 204:1837.
[10]. Rickel, E.A. et al. (2008) J. Immunol. 181:4299.
[11]. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
[12]. Kleinschek, M.A. et al. (2007) J. Exp. Med. 204:161
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Accession # |
Q8VHH8 |
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Alternate Names |
IL17E; IL-17E; IL25; IL-25; interleukin 25; Interleukin-17E; interleukin-25 |
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Source |
Human embryonic kidney cell, HEK293-derived mouse IL25/IL17E protein |
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Protein sequence |
Val17-Ala169 |
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M.Wt |
17.5 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 0.02-1.0 ng/mL. Measured by its ability to induce CXCL1/GRO alpha secretion in HT-29 human colon adenocarcinoma cells. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.