Recombinant Mouse Oncostatin(OSM), Tag Free
Oncostatin M (OSM) is a member of a cytokine subfamily that includes IL-6, IL-11, LIF, CNTF, and cardiotrophin-1. These cytokines have overlapping biological functions and shared receptor components. Mouse OSM was cloned and identified as an immediate early gene induced in various myeloid and lymphoid cell lines by a subset of cytokines including IL-2, IL-3, GM-CSF and EPO. The mouse OSM cDNA encodes a 263 amino acid residue precursor protein that shows 48% identity with human OSM. Similar to human OSM, the C-terminal region of mouse OSM contains a highly charged region. Deletion of this C-terminal region appears to be essential for the formation of biologically active mOSM.The biological activity of human OSM has been shown to be mediated either by the LIF/OSM receptor complex composed of gp130 and LIF R alpha or by a human OSM specific receptor composed of gp130 and OSM R alpha. It remains to be determined if the biological activities of mouse OSM can also be mediated by both receptor complexes in mouse cells.
Reference
[1]. Yoshimura, A. et al. (1996) The EMBO Journal 15:1055.
[2]. Ray, P. et al. (1996) Endocrinology 137:1151.
[3]. Rose, T.M. and A.G. Bruce (1994)Oxford University Press, New York, p. 127.
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Accession # |
P53347 |
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Alternate Names |
MGC20461; oncostatin M; oncostatin-M; OSM |
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Source |
Human embryonic kidney cell, HEK293-derived mouse Oncostatin M/OSM protein |
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Protein sequence |
Ala24-Arg206 |
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M.Wt |
20.5 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70°C as supplied. |
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Concentration |
0. 2 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
The EC50 for this effect is 0.02-1 ng/mL. Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. |
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Shipping Condition |
Shipping with dry ice. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 95%, determined by SDS-PAGE.
- Datasheet
Endotoxin: <0.010 EU per 1 ug of the protein by the LAL method.