Recombinant Murine Keratinocyte Growth Factor 1/FGF-7
Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, murine KGF-1 shares 96 % amino acid sequence identity with human and rat.
Reference
1. Mattei MG, deLapeyriere O, Bresnick J, et al. 1995. Mamm Genome. 6:196-7
2. Kelley MJ, Pech M, Seuanez HN, et al. 1992. Proc Natl Acad Sci U S A. 89:9287-91
3. de Giorgi V, Sestini S, Massi D, et al. 2007. Dermatol Clin. 25:477-85, vii
4. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49
5. Belleudi F, Leone L, Nobili V, et al. 2007. Traffic. 8:1854-72
6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7
7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.
| Gene ID | 14178 |
| Accession # | P36363 |
| Alternate Names | ,HBGF-7 |
| Source | Escherichia coli. |
| M.Wt | Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids. |
| AA Sequence | CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rMuKGF-1/FGF-7 as determined by LAL method.