Recombinant Rat Eotaxin-2/CCL24
Catalog No.
P1532
Featured Products
CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.
Reference
1. Owczarek W, Paplinska M, Targowski T, et al. 2010. Cytokine, 50: 181-5
2. Ben-Yehuda C, Bader R, Puxeddu I, et al. 2008. Exp Lung Res, 34: 467-79
3. Schaefer D, Meyer JE, Pods R, et al. 2006. Int Arch Allergy Immunol, 140: 205-14.
| Alternate Names | CK-beta-6, MPIF-2, Small-inducible Cytokine A24 |
| Source | Escherichia coli. |
| M.Wt | Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2. |
| AA Sequence | MPTGSVTIPS SCCVTFISKK IPVNRVISYQ LANGSICPKA GVIFITKKGH KICTDPKLPW VQKHIKNLDA KRNQPSEGAK ALGPKFVIQK LRGNSTKV |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
-
Purity > 96 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRtEotaxin-2/CCL24 as determined by LAL method.