Recombinant Rat Interleukin-2, Liquid
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.
Reference
1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79
2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10
3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51
4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25
5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.
| Gene ID | 116562 |
| Accession # | P17108 |
| Alternate Names | T-cell Growth Factor, CGF |
| Source | Escherichia coli. |
| M.Wt | Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids. |
| AA Sequence | APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ |
| Appearance | Sterile Colorless liquid. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 6 months from date of receipt, -20 to -70 °C as supplied. - 3 months, -20 to -70 °C under sterile conditions after opening. |
| Formulation | A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0, 30 % glycerol. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 97 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRtIL-2, Liquid as determined by LAL method.