Recombinant Rat PDGF-AA

Gene ID

Accession #

Alternate Names

Source

Escherichia coli.

M.Wt

Approximately 25.3 kDa, a disulfide-linked homodimeric protein containing two 111 amino acid residues polypeptide (A chain).

AA Sequence

MSIEEAIPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETDV R

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles
- 12 months from date of receipt, -20 to -70 °C as supplied
- 1 month, 2 to 8 °C under sterile conditions after reconstitution
- 3 months, -20 to -70 °C under sterile conditions after reconstitution

Formulation

Lyophilized from a 0.2 mm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 ×10⁵ IU/mg

Shipping Condition

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans.