Recombinant Rhesus Macaque Interleukin-3
Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology.
Reference
1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10
2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95
3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24
4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-4
1.
| Gene ID | 706946 |
| Accession # | P25140 |
| Alternate Names | Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor |
| Source | Escherichia coli. |
| M.Wt | Approximately 14.0 kDa, a single non-glycosylated polypeptide chain containing 124 amino acids. |
| AA Sequence | APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ |
| Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Biological Activity | Assay #1: Fully biologically active when compared to standard. The ED50as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 × 105IU/mg. Assay #2: The ED50as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg. |
| Shipping Condition | Gel pack. |
| Handling | Centrifuge the vial prior to opening. |
| Usage | For Research Use Only! Not to be used in humans. |
Quality Control & DataSheet
- View current batch:
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Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rRhIL-3 as determined by LAL method.