NHS-LC-LC-Biotin Kit
NHS-LC-LC-Biotin is a double-long-chain, NHS (N-hydroxysuccinimide) ester-activated, non-sulfonated biotinylation reagent for labeling primary amines (-NH2), such as protein lysine, that enables simple and efficient biotinylation of antibodies, proteins, and any other primary amine-containing biomolecules in solution for detection or purification using streptavidin probes or resins.
The NHS-LC-LC-Biotin Kit is a biotin labeling kit developed based on NHS-LC-LC-Biotin, which provides a complete set of reagents for biotin labeling. The biotin contained in the kit has been activated and ready for immediate use, with up to 1 mg of antibody labeled at a time. The kit also includes buffer, ultrafiltration tube, protective solution, antibodies do not need dialysis, easy to operate, and the complete process can be completed in only 90 minutes.
This kit can be used for biotin labeling of antibodies or proteins, and the labeled antibodies are tested for most ELISA applications and some IHC applications. Compared with other biotin labeling kits, the long-arm biotin kit labeled antibody has a higher signal-to-noise ratio in ELISA applications.
| Components | K4401-2 T | K4401-5 T |
| NHS-LC-LC-Biotin | 2X 0.1 mg | 5X 0.1 mg |
| Labeling Buffer | 15 mL | 30 mL |
| DMF | 200 μL | 200 μL |
| Ultrafiltration tube | 4X 0.5 mL | 10X 0.5 mL |
| Antibody Stabilization Solution | 1 mL | 2 mL |
Store the kit unopened at 2-8℃ for 1 year, and the dissolved biotin can be stored at 2-8℃ for 1 week. | ||