Cy3 NHS ester
Sulfo-Cy3 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For labeling proteins and peptides, no organic co-solvent is needed. Sulfo-Cy3 NHS ester is ideal for proteins with low solubility and proteins prone to denaturation. Non-sulfonated Cy3 NHS ester is also available. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In vitro, sulfo-Cy3 NHS ester can react with green-emitting QDs ligand-exchanged and LA/His-PIMA-PEG-NH2 to provide QD-dye conjugates [1].
Reference:
[1] Reid, D. A.; Keegan, S.; Leo-Macias, A.; Watanabe, G.; Strande, N.T.; Chang, H.H.; Oksuz, B.A.; Fenyo, D.; Lieber, M.R.; Ramsden, D.A.; Rothenberg, E. Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair. Proceedings of the National Academy of Sciences of the U.S.A., 2015, 112(20).
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 735.80 |
| Formula | C34H38N3NaO10S2 |
| Solubility | insoluble in EtOH; insoluble in DMSO; insoluble in H2O |
| Chemical Name | (2E)-1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-2-[(E)-3-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)prop-2-enylidene]indole-5-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=C3C(C4=C(N3CCCCCC(=O)ON5C(=O)CCC5=O)C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 563 | Emission max (nm) | 584 |
| Extinction Coefficient (M-1cm-1) | 162000 | Quantum Yield | 0.1 |
| CF260 | 0.03 | CF280 | 0.06 |
Quality Control & MSDS
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Chemical structure
Related Biological Data
Related Biological Data
