Cy5 azide
Sulfo-Cy5 azide is a sulfonated, hydrophilic and highly water soluble dye for Click chemistry. Due to this features, the dye can be used for labeling biomolecules, especially useful for the labeling of delicate proteins and proteins prone to denaturation, purely aqueous conditions. Sulfo-Cy5 azide is not only a sulfonated but also a popular fluorophore. For labeling proteins and peptides, no organic co-solvent is needed. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye–dye interactions.
In mammalian cells, Sulfo-Cy5-azide was incubated with U2OS cells. Finally, the study only find that the cells expressing tRNAIle2-5S fusion RNA can be labeled and imaged with Sulfo-Cy5-azide [1].
Reference:
[1] Li, F. ; Dong, J.; Hu, X.; Gong, W.; Li, J.; Shen, J.; Tian, H.; Wang, J. A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells. Angewandte Chemie International Edition, 2015, 54(15), 4597–4602.
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| M.Wt | 778.91 |
| Formula | C36H47N6NaO8S2 |
| Solubility | very high in water |
| Chemical Name | (2E)-1-[6-(3-azidopropylamino)-6-oxohexyl]-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethyl-5-sulfonatoindol-1-ium-2-yl)penta-2,4-dienylidene]indole-5-sulfonate |
| SDF | Download SDF |
| Canonical SMILES | CC1(C2=C(C=CC(=C2)S(=O)(=O)[O-])[N+](=C1C=CC=CC=C3C(C4=C(N3CCCCCC(=O)NCCCN=[N+]=[N-])C=CC(=C4)S(=O)(=O)[O-])(C)C)C)C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 646 | Emission max (nm) | 662 |
| Extinction Coefficient (M-1cm-1) | 271000 | Quantum Yield | 0.28 |
| CF260 | 0.04 | CF280 | 0.04 |
Quality Control & MSDS
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Chemical structure
Related Biological Data
