Cy5 NHS ester
Sulfo-Cy5 NHS ester is a sulfonated, hydrophilic and highly water soluble dye. For the labeling of various amine-containing molecules in aqueous phase, no organic co-solvent is needed. This regent is especially useful for the labeling of proteins which denaturate in the presence of organic co-solvents, as well as for proteins with low solubility. Sulfo-Cy5 NHS ester is one of the most popular fluorophores. In life science, the sulfonate groups make the Cy dyes soluble in water and also reduce fluorescence-quenching which can arise from dye-dye interactions.
Sulfo-Cy5 NHS ester, was conjugated to LLP2A with 646/662 peak excitation/emission in dimethyl sulfoxide. LLP2A exhibited punctate staining around the cell periphery, consistent with it labeling VLA-4 [1].
Reference:
[1] Soodgupta, D. ; Zhou, H.; Beaino, W.; Lu, L.; Rettig, M.; Snee, M.; Skeath, J.; DiPersio, J.F.; Akers, W.J.; Laforest, R.; Anderson, C.J.; Tomasson, M.H.; Shokeen, M. Ex Vivo and In Vivo Evaluation of Overexpressed VLA-4 in Multiple Myeloma Using LLP2A Imaging Agents. Journal of Nuclear Medicine, 2016, 57(4), 640–645.
- 1. Guo M, Du W, et al. "Ultra-Early Diagnosis of Acute Myocardial Infarction in Rats Using Ultrasound Imaging of Hollow Double-Layer Silica Nanospheres." Adv Healthc Mater. 2020;9(3):e1901155. PMID:31867893
- 2. Hu Y, Hou Y, et al. "Polysarcosine as an Alternative to PEG for Therapeutic Protein Conjugation." Bioconjug Chem. 2018 Jul 18;29(7):2232-2238. PMID:29863329
- 3. Batzaya Nyambat, Chih-Hwa Chen, et al."Genipin-Crosslinked Adipose Stem Cell Derived Extracellular Matrix-Nano Graphene Oxide Composite Sponge for Skin Tissue Engineering." Journal of Materials Chemistry B. 2018 January 15.
| Storage | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| M.Wt | 761.84 |
| Formula | C36H40NaN3O10S2 |
| Solubility | insoluble in H2O; insoluble in EtOH; insoluble in DMSO |
| SDF | Download SDF |
| Canonical SMILES | CC(C1=CC([Na])=CC=C1N/2C)(C)C2=C/C=C/C=C/C3=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=CC=C(S(=O)([O-])=O)C=C5C3(C)C.O=S(=O)=O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Excitation max (nm) | 646 | Emission max (nm) | 662 |
| Extinction Coefficient (M-1cm-1) | 271000 | Quantum Yield | 0.28 |
| CF260 | 0.04 | CF280 | 0.04 |
Quality Control & MSDS
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Chemical structure
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