Sulfo-NHS-Biotin Kit (90-180 kDa)

Sulfo-NHS-Biotin Kit (90-180 kDa) is a kit that labels proteins such as antibodies with amino groups with biotin. Biotin molecules modified with active esters in the kit can specifically covalently label molecules such as amino-containing proteins to achieve biochemical modification of proteins and subsequent immunolabeling reactions. When this product is used to label proteins, especially immunoglobulin G (IG G), the Filtration Tube included in the kit can be used to remove inhibiting labeled reactants or low molecular weight compounds such as unreacted biotin, such as Tris, etc., to increase the labeling effect. Compared to conventional treatments such as dialysis and gel filtration, this kit results in higher recovery and higher purity of Biotin-labeled compounds.

This product is easy to operate, only ammonia-reactive biotin components need to be added to IgG and other protein antibody solutions, and incubated at 37°C for 10-15 min to achieve an average of 5-8 biotin molecular marker binding per IgG molecule, and the whole process can be completed in about 1 h. This product is only suitable for 90-180 kDa, 50-200 μg of protein molecular markers.