T7 RNA Polymerase (GMP-grade)

T7 RNA Polymerase is a DNA-dependent RNA polymerase with a molecular weight of 99 KD, and high specificity for the T7 phage promoter. T7 RNA polymerase uses double-stranded DNA containing the T7 promoter sequence as a template and NTP as a substrate to synthesize RNA complementary to the reverse single-stranded DNA downstream of the promoter. Double-stranded linear blunt-end or 5' sticky-end DNA can be used as a substrate template for T7 RNA polymerase, so linear plasmids and PCR products can be used as templates for in vitro synthesis of RNA.

RNA synthesized by T7 RNA polymerase covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots. This product is a bacteriophage T7 RNA polymerase derived from the recombinant expression of E. coli, and the production process strictly controls host protein residues, nucleic acid residues, etc., in line with GMP production and quality management practices.