T7 RNA Polymerase

This product is an E. coli recombinant expressed phage T7 RNA polymerase, a DNA-dependent RNA polymerase with high specificity for the T7 phage promoter. This 99 KD T7 RNA polymerase uses double-stranded DNA containing the T7 promoter sequence as a template and NTP as a substrate to synthesize RNA complementary to the reverse single-stranded DNA downstream of the promoter. Double-stranded linear blunt-end or 5'sticky-end DNA can be used as a substrate template for T7 RNA polymerase, so linear plasmids and PCR products can be used as templates for in vitro synthesis of RNA.

RNA synthesized by T7 RNA polymerase covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.