TNF-alpha, recombinant murine protein
Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Reference:
1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9
2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483
3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41
4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.
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Gene ID |
21926 |
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Accession # |
P06804 |
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Alternate Names |
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin |
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Source |
Escherichia coli. |
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M.Wt |
Approximately 17.4 kDa. The recombinant murine TNF-α is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein. |
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AA Sequence |
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL |
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Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
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Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.2. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg in the presence of actinomycin D. |
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Shipping Condition |
Gel pack. |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
- 1. Breno Emanuel Farias Frihling, Ana Paula de Araújo Boleti, et al. "Purification, Characterization and Evaluation of the Antitumoral Activity of a Phospholipase A2 from the Snake Bothrops moojeni." Pharmaceuticals (Basel). 2022 Jun 7;15(6):724. PMID: 35745643
- 2. Qionghui Zhan, Jaepyo Jeon, et al. "CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux." Autophagy. 2022 Apr;18(4):726-744. PMID: 34282994
- 3. Rossi A, Pakhomova ON, et al. "Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors." Sci Rep. 2019 Jan 23;9(1):431. PMID: 30674926
Quality Control & DataSheet
- View current batch:
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Purity > 98 % by SDS-PAGE and HPLC analyses.
- Datasheet
Endotoxin: Less than 1 EU/μg of rMuTNF-α as determined by LAL method.
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