TNF-alpha, recombinant rat protein
Catalog No.
P1003
TNF-α, expressed in E. coli, suitable for cell culture.
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Rat tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a transmembrane protein with 235 aa that is cleaved to the soluble monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, viral replication, tumorigenesis, apoptosis, cell proliferation, differentiation, coagulation and lipid metabolism. TNF-α is mainly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells.
| Determined by the cytolysis of murine L929 cells in the presence of actinomycin D. | ||||||
| ED50 | < 0.05 ng/ml. | |||||
| Specific activity | > 2*107 units/mg. | |||||
Quality Control & DataSheet
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Purity: ≥95%, by SDS-PAGE and HPLC.
Endotoxin Level:<0.1 ng/μg
Related Biological Data
Related Biological Data
| Gene ID: | 24835 | Accession #: | P16599.1 |
| Alternate Names: | TNFA, TNF alpha; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha. | ||
| Source: | Escherichia Coli. | M.Wt: | 17 kDa |
| Appearance: | Lyophilized powder | Storage: | Store at -20°C |
| Formulation: | Sterile filtered and lyophilized from 1× PBS. | ||
| Solubility: | Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4℃ for 1 week or -20℃ for future use. | ||
| Shipping Condition: | Gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||