Laminin (925-933)
Laminin (925-933) is a peptide(Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg) derived from residues 925-933 of the laminin B1 chain that binds to the laminin receptor.
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively)
The laminin beta 1 chain has 7 structurally distinct domains, which it shares with other beta chain isomers. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.
Figure1 Structure of Laminin
Figure2 Formula of LamininB1 (925-933)
Ref:
1.Ikonen J, Pikkarainen T, Savolainen ER, Tryggvason K (Feb 1989). "A Hpa I polymorphism in the human laminin B1 chain gene on 7q22". Nucleic Acids Res 17 (1): 473.
2.Roche KB, Moore JW, Surana RB, Wilson BE (May 1989). "Aortic root dilatation associated with partial trisomy 7(q31.2----qter)". Pediatr Cardiol 10 (1): 53–5.
3.Bonneau D, Huret JL, Godeau G, Couet D, Putterman M, Tanzer J, Babin P, Larregue M (Sep 1991). "Recurrent ctb(7)(q31.3) and possible laminin involvement in a neonatal cutis laxa with a Marfan phenotype". Hum Genet 87 (3): 317–9.
- 1. Talbert, Lance Edward. "Advancing Academics Through Laboratory and Classroom Research: Probing Biomolecular Structure with Novel Mass Spectrometry Methods and Revisiting Student Learning Tools." UNIVERSITY OF CALIFORNIA. June 2019.
- 2. Lance E.Talbert, XingZhang, et al. "Synthesis of New S-S and C-C Bonds by Photoinitiated Radical Recombination Reactions in the Gas Phase." International Journal of Mass Spectrometry. Available online 5 April 2019.
- 3. Talbert LE, Julian RR. "Directed-Backbone Dissociation Following Bond-Specific Carbon-Sulfur UVPD at 213 nm." J Am Soc Mass Spectrom. 2018 Apr 5. PMID:29623659
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 967.06 |
| Cas No. | 110590-60-8 |
| Formula | C40H62N12O14S |
| Synonyms | H2N-Cys-Asp-Pro-Gly-Tyr-Ile-Gly-Ser-Arg-OH |
| Solubility | ≥15.53 mg/mL in H2O; ≥17.77 mg/mL in EtOH; ≥48.35 mg/mL in DMSO |
| Chemical Name | Laminin (925-933) |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C(C(=O)NCC(=O)NC(CO)C(=O)NC(CCCN=C(N)N)C(=O)O)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)CNC(=O)C2CCCN2C(=O)C(CC(=O)O)NC(=O)C(CS)N |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
HT-1080 and CHO cells, as well as B16F10 murine melanoma cells |
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Reaction Conditions |
100 and 300 µg/ml laminin (925-933) |
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Applications |
Laminin (925-933) stimulated the attachment of HT-1080 and CHO cells to culture plates when used at concentrations of 100 and 300 µg/ml for 1 h incubation. In addition, laminin (925-933) (100 µg/ml; 5 h incubation) was found to be a chemoattractant for the B16F10 murine melanoma cells, showing about 30% of the maximal response observed with full-length laminin. Laminin (925-933) could compete with full-length laminin and inhibit chemotactic response of the cells to full-length laminin. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Graf J, Iwamoto Y, Sasaki M, et al. Identification of an amino acid sequence in laminin mediating cell attachment, chemotaxis, and receptor binding. Cell, 1987, 48(6): 989-996. |
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Quality Control & MSDS
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Chemical structure
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