TNF-alpha, recombinant human protein
Tumor Necrosis Factor-α (TNF-α) is a potent cytokine expressed as a 26 kDa transmembrane protein with 233 aa that is cleaved to the soluble 17 kDa monomer by TNF-α converting enzyme (TACE) and forms stable homotrimers. TNF-α is expressed in many cells and plays an important role in inflammation, immune modulation, septic shock, viral replication, antitumor activity, anorexia, cachexia and hematopoiesis. TNF-α is chiefly produced by macrophages when challenged with viruses or bacteria. TNF-α is cytotoxic for many tumor cells lines but induced activation, differentiation or proliferation in normal cells. Tumor necrosis factor receptor TNFR1 and TNFR2 are receptors for TNF-α.
- 1. Qionghui Zhan, Jaepyo Jeon, et al. "CAMK2/CaMKII activates MLKL in short-term starvation to facilitate autophagic flux." Autophagy. 2022 Apr;18(4):726-744. PMID:34282994
- 2. Liu X, Zhang X, et al. "Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells." J Cell Mol Med. 2019 Sep 29. PMID:31565867
- 3. Rossi A, Pakhomova ON, et al. "Mechanisms and immunogenicity of nsPEF-induced cell death in B16F10 melanoma tumors." Sci Rep. 2019 Jan 23;9(1):431. PMID:30674926
| Storage | Store at -20°C or -70°C |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
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Cell lines |
Human neuroblastoma SH-SY5Y cells |
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Reaction Conditions |
20 ng/ml TNF-α for 24 h incubation |
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Applications |
TNF-α upregulated CXCR4 expression in neuroblastoma cells and increased migration to the CXCR4 ligand SDF‑1α. In addition, this effect was dependent upon NF-κB transcriptional activity, as blocking the NF‑κB pathway with pyrrolidinedithiocarbamic acid ammonium salt suppressed TNF‑α‑induced upregulation of CXCR4 expression and reduced the migration towards the CXCR4 ligand, SDF‑1α. These findings indicate that the inflammatory cytokine, TNF‑α, partially functions through the NF‑κB signaling pathway to upregulate CXCR4 expression to foster neuroblastoma cell metastasis. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Zhi Y, Lu H, Duan Y, et al. Involvement of the nuclear factor-κB signaling pathway in the regulation of CXC chemokine receptor-4 expression in neuroblastoma cells induced by tumor necrosis factor-α. International Journal of Molecular Medicine, 2015, 35(2): 349-357. |
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| Determined by the cytolysis of murine L929 cells in the presence of actinomycin D. | ||||||
| ED50 | < 1 ng/ml. | |||||
Quality Control & DataSheet
- View current batch:
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Purity: > 95% by SDS - PAGE.
- COA (Certificate Of Analysis)
- Datasheet
Endotoxin Level: N/A
Related Biological Data
Related Biological Data
Related Biological Data
| Gene ID: | 7124 | Accession #: | P01375 |
| Alternate Names: | TNFA, human TNF-alpha, Human TNF-α, Tumor Necrosis Factor-alpha | ||
| Source: | E.coli | M.Wt: | 17.4 kDa |
| Appearance: | Lyophilized powder | Storage: | Store at -20°C or -70°C |
| Formulation: | Lyophilized from 0.22 μm filtered PBS (pH 7.4) with 5% trehalose | ||
| Solubility: | Reconstitute in H2O to a concentration of 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. | ||
| Shipping Condition: | gel pack. | Handling: | Centrifuge the vial prior to opening. |
| Usage: | For Research Use Only! Not to be used in humans. | ||