Cytochrome P450 CYP1B1 (190-198) [Homo sapiens]

H2N-FLDPRPLTV-OH

Cytochrome P450 CYP1B1 is a dioxin-inducible form of the cytochrome P450 family of xenobiotic metabolizing enzymes¹.

HLA-A2 restricted, CYP1B1 190–198 (CYP190) peptide specific T cell responses are induced in HLA-A2K^b transgenic mice by immunization with ZYC300, a PLG microparticle formulation that contains a plasmid DNA encoding the tumor associated antigen CYP1B1^{2, 3}.

Each successive co-application of EPT contributes to an escalating T cell response that can be observed 2 weeks after the last immunization, and that even a single EPT when given with the usually ineffective 30 µg dose of ZYC300 was able to induce a detectable CD8⁺ CYP190 specific T cell response⁴.

Figure1. Cytochrome P450 CYP1B1

Figure2. Formula of Cytochrome c fragment (93-108)

C₅₀H₈₀N₁₂O₁₃

Figure3.Location of Cytochrome P450 CYP1B1

The CYP1B1 gene is located on the short (p) arm of chromosome 2 at position 22.2.

Ref:

1. Murray,G.I., Taylor, M. C., et al 1997; Tumor-specific Expression of Cytochrome P450 CYP1B11. Cancer Research. 57:3026-3031.

2. Luby TM, Cole GA, Baker L, Kornher JS, Ramstedt U, Hedley ML. Repeated immunization with plasmid DNA formulated in poly(lactide-co-glycolide) microparticles is well tolerated and stimulates durable T cell responses to the tumor associated antigen cytochrome P450 1B1. Clinical Immunology 2004;112(1):45–53.

3. Maecker DH, Sherr RH, Vonderheide MS, von Bergwelt-Baildon MS, Hirano N, Anderson K, et al. The shared tumor-associated antigen cytochrome P450 1B1 is recognized by specific cytotoxic T cells. Blood 2003;102(9):3287–94.

4. Barbon, C. M., Baker, L et al 2010 In vivo electroporation enhances the potency of poly-lactide co-glycolide (PLG) plasmid DNA immunization. Vaccine; 28:7852-7864